Murashige and Skoog (MS) Modified Wheat Regeneration MediumRegeneration medium for monocots Contains MS basal salts with vitamins, sucrose and agar to be supplemented with specific growth regulators. Add 0. 001 g L IAA and 0. 002 g L 6 BAP after autoclaving Provided as 1 unit to make 10L, 25L, or 50L of medium

Purified high gel strength agar with excellent properties for tissue culture and microbiological work

Spray Bottle (cat# 21530103-1)

Phytoagar may be added at 8-15 g/L before autoclaving for use as a solid agar growth medium

May be followed by Stage II: 30630174 - Rose Modified Multiplication Basal Medium

bioWORLD offers a high quality Protoplast Regeneration Medium

The solution does not contain DMF or DMSO

See Antifungal Treatments - Continuous Infusion by PubMed

Moistures - 10

Bacteriological Agar may be added at 15 g/L before autoclaving

One unit of Cellulase is observed to liberate 1 µMole of Glucose from Carboxymethyl Cellulose

See 'Simplified Arabidopsis Transformation Protocol' by Arabidopsis

filtered sterilized 10 mL of 100X vitamin stock solution